The research activities of this area at the cross-roads of materials science, nanotechnology and cell biology, primarily focusing on how newly-discovered materials and interfacial processes can be developed and used for specific applications. In particular, our research emphasizes cytomechanic studies correlating cytomechanical profiles to the underlying molecular and cellular mechanisms to isolate and study extracellular vesicles, with materials engineering promoting and driving cell adhesion and differentiation.
Cellular homeostasis and cytomechanics
The surface of living cells is strictly related to many cellular processes such as adhesion, signalling, transport, energy transformation, tumour metastasis etc. Cellular functions are mediated by a plethora of specific biomolecules including cell-to-cell adhesion proteins and those that constitute the highly complex and dynamic architecture of the cytoskeleton which, in turn, connects structurally and functionally the intracellular environments with the extracellular matrix via other adhesion molecules.
Our research activities focus on the biomolecular and cytomechanical cellular behaviour of primary fibroblast cultures related to juvenile hereditary Parkinson’s disease and of epithelial tumour cell lines. Nanomechanical properties of single cells obtained by advanced microscopic techniques allow to correlate morpho-mechanical cellular state changes upon interaction with pharmacological agents and the underlying biological \ biochemical processes.
Analysis of Extracellular vesicles
A recently developed research activity concerns the EVs isolation from fibroblasts of parkin-mutant patients and the characterization of their biochemical differences compared to EV derived from control samples. In particular, EVs are isolated from culture media by an optimized differential centrifugation protocol and their size and morphology determined by flow cytometry, electron and atomic force microscopy. We are also performing a mass spectrometry analysis to investigate their lipidomic profiles.
Materials for cell engineering
Cell engineering is a very promising research field which aims to induce specific biological processes such as proliferation and differentiation, cell-to-cell interaction, biomolecular production and extracellular matrix (ECM) formation, at the cell/material interface. Specifically cell migration is a phenomenon that is involved in different physiological processes such as morphogenesis, wound healing and tumour invasion. Biochemical or biophysical stimuli such as chemotaxis, galvanotaxis extracellular matrix compliance/stiffness and topology can influence cell migration in terms of speed, direction and persistence. The control of the cell environment by multiple physicochemical cues has therefore emerged as a key factor to enable functionality, modulate response, and affect cell behaviour.
In particular our recent studies have shown that cells are able to recognize the mechanical properties of a substratum over which they move and that these properties direct the motion through a phenomenon called durotaxis. Thus, mechanical interactions between a cell and its underlying substratum play a crucial role in modulating cell motility. We are investigating combinations of external stimuli ranging from chemical to mechanical and electrical cues in the cellular microenvironment. Moreover we are carrying out cell tracking analysis to deepen our understanding of the mechanisms underlying cell motility.
Plasma processing to control cell adhesion
The nature of the interface between cells and materials can stimulate a repulsive (or adhesive) response that can causes the cells to separate (adhere) to the desired material. This effect dramatically depends on surface properties of the material. Plasma surface modification is applicable whenever the surface of the device has to be bioactive or bioinert by changing chemical/topographical features of a material surface without affecting its bulk properties. As an example, the irreversible, undesired adhesion of biomolecules and cells (i.e. ‘biofouling’) can be controlled by a plasma assisted deposition of Polyethylene oxide (PEO)-like coatings. On the other hand, surfaces containing polar groups (i.e NH2, COOH, OH etc. …) or deposition of coatings in which biological molecules are dispersed in an organic matrix can be produced by plasma in order to impart bioactivity and biocompatibility to the surface. Finally, surfaces with micro- and nanostructured coatings can dramatically improve cell/material interactions due to topographical cues.
Plasma processing of cells and biological liquids
Cold atmospheric pressure plasmas are emerging as an exciting development for therapeutics. These plasmas are very efficient sources of highly reactive oxygen and nitrogen species (RONS), UV radiation, electromagnetic fields and charged particles. Experiments show that cold atmospheric plasmas allow efficient, contact-free and painless disinfection, without damaging healthy tissue. In healthcare, new horizons are being opened for wound healing, tissue regeneration, cancer therapy, and treatment of chronic wounds assisted by plasma technology.
Our facilities have been designed to allow us to study the response of different type of cells to different plasma doses and to correlate the chemical composition of plasma treated cell culture media with cell behaviour.
- Lippolis, R. A. Siciliano, C. Pacelli, A. Ferretta, M. F. Mazzeo, S. Scacco, F. Papa, A. Gaballo, C. Dell’Aquila, M. De Mari, S. Papa , T. Cocco, Altered protein expression pattern in skin fibroblasts from parkin-mutant early-onset Parkinson’s disease patients. Biochimica Biophysica Acta, 1852, 1960-1970 (2015). ISSN: 0925-4439; doi: 10.1016/j.bbadis.2015.06.015
- Vergara, P. Simeone, F. Julien, M. Trerotola, A. Giudetti, L. Capobianco, A. Tinelli, C. Bellomo,
I. Fournie, A. Gaballo, S. Alberti, M. Salzet, M. Maffia, Translating epithelial mesenchymal transition markers into the clinic: Novel insights from proteomics EuPA Open Proteomics, 10, 31-41, (2016) ISSN: 2212-9685; doi:10.1016/j.euprot.2016.01.003
- Vergara, M. Ferraro, M.F. Cascione, L.L. del Mercato, S. Leporatti, A. Ferretta, P. Tanzarella, C. Pacelli, A. Santino, M. Maffia, T. Cocco, R. Rinaldi, A. Gaballo, Cytoskeletal Alterations and Biomechanical Properties of parkin-Mutant Human Primary Fibroblasts. Cell Biochemistry Biophysics, 71, 1395-1404, (2015). ISSN:1085-9195; doi: 10.1007/s12013-014-0362-1
- Ferretta, A. Gaballo, P. Tanzarella, C. Piccoli, N. Capitanio, B. Nico, T. Annese, M. Di Paola, C. Dell’aquila, M. De Mari, E. Ferranini, V. Bonifati, C. Pacelli, T. Cocco, Effect of resveratrol on mitochondrial function: implications in parkin-associated familiar Parkinson’s disease Biochimica Biophysica Acta, 1842, 902-915 (2014). ISSN: 0925-4439; doi: 10.1016/j.bbadis.2014.02.010
- Vergara, P. Simeone, D. Latorre, M.F. Cascione, S. Leporatti, M. Trerotola, A.M. Giudetti, L. Capobianco, P. Lunetti, A. Rizzello, R. Rinaldi, S. Alberti, M. Maffia. Proteomics analysis of E-Cadherin knockdown in epithelial breast cancer cells J. Biotechnology Special Issue EuroBiotech 22, 3-11 (2015). ISSN: 0168-1656; doi:10.1016/j.jbiotec.2014.10.034.
- E Palamà, S. D’Amone, B. Cortese, Chapter 22: Mechanical guidance of cell migration, Nanomaterials and Regenerative Medicine (Y. Lin and T. Gong, ed.) IAPCOBP Publishing, (2016) (invited chapter). ISBN: 978-953-56942-3-6. DOI: 10.5599/obp.9.0
- Pagani, R. C. Paolicelli, E. Murana, B. Cortese, S. Di Angelantonio, E. Zurolo, E.Guiducci, T. A. Ferreira, S. Garofalo, M. Catalano, G. D’Alessandro, A. Porzia, G.Peruzzi, F. Mainiero, C. Limatola, C.T. Gross, D. Ragozzino, Defective microglial development in the hippocampus of CX3CR1 deficient mice Front. Neurosci. 9,111. (2015) ISSN: 1662-5102; doi: 10.3389/fncel.2015.00111. ISSN: 1662-453X.
- Cortese, I.E Palamà, S. D’Amone, G. Gigli. Influence of electrotaxis on cell behaviourIntegr.Biol, 6,817 – 830, (2014). ISSN: 1520-6602; doi: 10.1039/C4IB00142G Integrative Biology 2014 HOT Articles.
- Sardella, E.R. Fisher, J.C. Shearer, M.G. Trulli, R. Gristina, P. Favia. N2/H2O plasma assisted functionalization of Poly(epsilon-caprolactone) porous scaffolds: acid/basic character versus cell behavior. Plasma processes and Polymers 12-8, 786-798 (2015). ISSN: 1612-8850; doi: 10.1002/ppap.201400201
- Yang, G. Camporeale, E. Sardella, G. Dilecce, J.S. Wu, F. Palumbo, P. Favia; Deposition of Hydroxyl Functionalized Films by means of water Aereosol assisted Atmospheric pressure plasma Plasma processes and polymers 11-11, 1102-1111 (2014) ISSN: 1612-8850; doi: 10.1002/ppap.201400066
- Trizio, E. Sardella, E. Francioso, G. Dilecce, V. Rizzi, P. Cosma, M. Schmidt, M. Hansch, T. von Woedtke, P. Favia, R. Gristina; Investigation of air-DBD effects on biological liquids for in vitro studies on eukaryotic cells, Clinical Plasma Medicine 3-2, 62-71 (2015). ISSN: 22128166; doi: 1016/j.cpme.2015.09.003.
Other selected publications
- C. Pacelli, D. De Rasmo, A. Signorile, I. Grattagliano, G. di Tullio, A. D’Orazio, B.Nico, G. P. Comi, D. Ronchi, E. Ferranini, D. Pirolo, P. Seibel, S. Schubert, A. Gaballo, G. Villani, T. Cocco Mitochondrial defect and PGC-1α dysfunction in parkin-associated familial Parkinson’s disease. Biochimica Biophysica Acta, 1812,1041-1053, (2011) ISSN: 0925-4439; doi: 10.1016/j.bbadis.2010.12.022
- N. Denora, V. Laquintana, A. Lopalco, R. M. Iacobazzi, A. Lopedota, A. Cutrignelli, G. Iacobellis, C. Annese, M. F. Cascione, S. Leporatti, M. Franco In vitro targeting and imaging the translocator protein TSPO 18-kDa through G(4)-PAMAM-FITC labeled dendrimers J. Contr. 172, 1111-1125 (2013). ISSN: 0168-3659; doi: 10.1016/j.jconrel.2013.09.024
- D. Vergara, P. Simeone, D. Toraldo, P. del Boccio, V. Vergaro, S. Leporatti, D. Pieragostino, A. Tinelli, S. De Domenico, S. Alberti, A. Urbani, M. Salzet, A. Santino, and M. Maffia Resveratrol downregulates Akt/GSK and ERK signalling pathways in OVCAR-3 ovarian cancer cells Molecular BioSystems 8, 1078-1087 (2012). ISSN: 1742-206X; doi: 10.1039/c2mb05486h
- Leporatti, D. Vergara, A. Zacheo, V. Vergaro, G. Maruccio, R. Cingolani, R. Rinaldi Cytomechanical and topological investigation of MCF-7 cells by scanning force microscopyNanotechnology 20 055103 (2009). (Paper of the month, Cover Page Issue) ISSN: 0957-4484; doi: 10.1088/0957-4484/20/5/055103
- I.E. Palamà, S. D’Amone, A.M.L. Coluccia, G. Gigli. Micropatterned polyelectrolyte multilayer films promote alignment and myogenic differentiation of C2C12 cells in standard growth medium. Biotechnology & Bioengineering, 110, 586-596, (2013). ISSN: 1097-0290; doi: 10.1002/bit.24626.
- B. Cortese, M.O. Riehle, S. D’Amone, G. Gigli, Influence of Variable Substrate geometry on Wettability and Cellular Responses, Journal of Colloid and Interface Science 394, 582–589, (2013). ISSN: 0021-9797, doi: 10.1016/j.jcis.2012.11.051.
- I.E. Palamà, S. D’Amone, A.M.L. Coluccia, M. Biasiucci, G. Gigli Cell self-patterning on uniform PDMS-surface with controlled mechanical cues Integrative Biology, 4, 228-236, (2012). doi: 10.1039/c2ib00116k; ISSN 1757-9708; (Highlight: the paper is the 5th position of the “top ten most accessed articles” of Integrative Biology web site in the month of December 2011)
- B. Cortese, G. Gigli, M. Riehle, Mechanical Gradient Cues for Guided Cell Motility and Control of Cell Behaviour on uniform substrates, Adv. Funct. Mater., 19, 2961-2968, (2009). ISSN: 1616-3028; doi: 10.1002/adfm.200900918;
- B. Cortese, C. Piliego, I. Viola, S. D’Amone, R. Cingolani, G. Gigli, Engineering transfer of micro and nanometer scale features by surface energy modification, Langmuir, 25, 7025–7031, (2009). ISSN 0743-7463; doi: 10.1021/la900248j.
- G. Da ponte, E. Sardella, F. Fanelli, R. d’Agostino, R. Gristina, P. Favia; Plasma deposition of PEO-like coatings wirth aereosol assisted dielectric barrier discharges PLASMA PROCESSES AND POLYMERS 9-11 1176-1183 (2012). ISSN: 16128850; doi: 10.1002/ppap.201100201
Process for the production by plasma of nanometric thickness coatings allowing controlled release of silver ions of other elements, or of molecules of biomedical interest, from solid products, and products thus coated R. D’agostino, P. Favia, F. Fracassi, E. Sardella, C. Costagliola, A. Mangone. Patent WO2013021409-A1: E. Sardella, P. Favia et al. WO2013021409 (2013)
Abstract: Process for the production by plasmochemical deposition of a film having a nanometric thickness, optionally multilayered, permitting carrying out in a controlled, uniform and long lasting way, release of substances of interest in a surrounding medium containing liquids, from a substrate including the substance to be released as micro/nano particles, or from a layer deposited on the substrate including the substance to be released as micro/nano particles, or from a layer of the substance to be released deposited on the substrate, or from a substrate that is the substance to be released optionally in the form of particles. The substances to be released can be metals, compounds having anti-bacterial properties, biologically active molecules such as drugs, hormones, vegetable extracts, peptides, lipids, protides and glucides. The layer with the substance to be released, be it organic or inorganic, is obtained by plasmochemical deposition optionally having a structure similar to polyethylene oxide (PEO) or polyethylene glycol (PEG), called PEO-like polymers, constituted, in a variable percentage da ethylene oxide units (-CH2CH2O-, EO); barrier film is obtained by depositing by plasma at least one organic or inorganic layer, optionally with a PEO-like structure, wherein chemical composition, degree of crosslinking and thickness are adjustable by the plasmo chemical deposition process parameters, and allow to adjust the release of the active substance according to specific needs. The structures on which the above said films can be deposited are: medical-surgical devices, common handworks, structures known as scaffolds, and the above defined substances to be released themselves. The invention also relates to medical-surgical devices, common handworks and scaffolds coated by a substrate and barrier layer, as well as to biologically active substances coated by at least one barrier layer.